Gene editing for improvement in plant and animal species

Code:
ATN/RF-18757-RG
Initiative:
Joint proyects
FONTAGRO Amount:
USD 230.000
Counterpart Amount:
USD 913.163
Execution time
42 Months
Total Amount:
USD 1.143.163
+
Participating countries:
Argentina Argentina Chile Chile Colombia Colombia Ecuador Ecuador Paraguay Paraguay Uruguay Uruguay Brazil Brazil
Funding source:
FONTAGRO Amount 20% Other agencies 0% Counterpart Amount 80%

Executive Summary

Crop and animal breeding is one of the constant challenges faced by the agricultural sector when trying to achieve increases in product yield and quality. The FAO proposes that, in order to meet nutritional demand during the next 32 years, the agricultural sector must produce between 50-70% more food, forage and biofuels than a decade ago. CRISPR-based gene editing (GE) is one of the most promising new biotechnologies for achieving this goal. GE is strongly based on knowledge of crop and animal gene functioning, and can be useful for conventional selection for breeds or varieties with desirable or novel traits in a short period of time. The enormous potential of this technique can be seen through proof of concept in the cultivation of important crops such as corn, cotton, rice, potato, soybean, and in cattle, sheep and pig breeding. The objective of this project is to consolidate regional GE research and innovation capacities for the improvement of species of agricultural interest, as a contribution to the technological sovereignty, value-added production, and the productive, economic and social development of the region. 

The technological solution

The technological solutions that the project is developing are:


a) The development of potato varieties with improved health, nutritional, and industrial quality enables more efficient and sustainable production, reducing the impact of diseases and enhancing their nutritional value and processability.
b) In the field of animal nutrition, new soybean varieties have been optimized for monogastric consumption, including pigs, poultry, and fish, with improved digestibility and a higher supply of essential amino acids, promoting their growth and feed conversion.
c) Genetic improvement in animal lines has led to the development of specific traits that optimize livestock production, such as greater feed conversion efficiency, disease resistance, and products with an improved nutritional profile, benefiting both producers and consumers.

Results

The gene editing project funded by FONTAGRO has made significant advances in improving crops and animal species through the application of the CRISPR/Cas9 system. In the agricultural sector, a didactic guide with a graphic and pedagogical approach was developed and distributed to facilitate the understanding of this technology, focusing on asexual reproduction crops such as potatoes. Additionally, protoplast regeneration and transfection processes were optimized, accelerating the development of improved varieties with greater genetic precision. These advancements not only contribute to food security and climate change adaptation but also strengthen the training of researchers in Latin America, consolidating scientific cooperation in the region.


In the livestock sector, the study enabled the design and selection of specific sgRNA guides for editing the bovine BLG gene to produce hypoallergenic milk, as well as the MSTN gene in cattle and sheep to enhance meat productivity. High efficiency was achieved in identifying conserved regions and minimizing undesired genomic effects, ensuring the safety and precision of the process. Furthermore, an optimized protocol for the in vitro production of genetically edited bovine embryos was developed, successfully reducing fertilization time without affecting embryonic development. This represents a key step in implementing biotechnology for genetic improvement in livestock farming in the region.

These results highlight the potential of gene editing to transform agricultural production, offering innovative and sustainable solutions to address global challenges such as food security and climate change. The optimization of protocols and the training of researchers ensure the effective transfer of knowledge, facilitating the adoption of this technology in agriculture and livestock farming. The dissemination of these achievements and the integration of new biotechnological tools will strengthen production systems in Latin America, promoting greater competitiveness and sustainability in the agricultural sector.

Beneficiaries

The potential beneficiaries of the genome editing project in plants and animals in South America are diverse and encompass various sectors and interest groups. Firstly, farmers and ranchers in the region will directly benefit from improved crops and animals offering higher yields, disease resistance, and nutritional quality, leading to increased productivity and profitability of their operations. Additionally, the agri-food industry will benefit from access to higher-quality raw materials with specific characteristics, potentially leading to the creation of new products and markets. Moreover, consumers will benefit from access to more nutritious and safer foods. Furthermore, regional scientific research and the academic community will benefit from strengthening their capacities in genome editing, promoting the advancement of knowledge and innovation in agricultural biotechnology in theses and other productive species of interest. In summary, the project has the potential to positively impact the entire agri-food chain, from producers to end consumers, contributing to the economic, social, and scientific development of the region.

Sustainable Development Goals

No poverty Zero Hunger Good health and well-being Responsible consumption and production Climate action Partnerships for the goals

Main donors

Participating Organizations

Executor
  • Fundación ArgenINTA (ARGENINTA) - Argentina
Co-executor
  • Instituto Nacional de Tecnología Agropecuaria (INTA) - Argentina
  • Instituto Nacional de Investigación Agropecuaria (INIA) - Uruguay
  • Instituto de Investigaciones Agropecuarias (INIA) - Chile
  • Instituto Paraguayo de Tecnología Agraria (IPTA) - Paraguay
  • Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA) - Colombia
  • Instituto Nacional de Investigaciones Agropecuarias (INIAP) - Ecuador
Associated
  • Empresa Brasileña de Investigación Agropecuaria (EMBRAPA) - Brasil
  • UdelaR - Uruguay
  • Asociados Don Mario SA (Asociados Don Mario SA ) - Argentina
  • Universidad de Buenos Aires (UBA) - Argentina
  • Programa Cooperativo para el Desarrollo Tecnológico Agroalimentario y Agroindustrial del Cono Sur (PROCISUR) - Uruguay
  • Asociación de Cooperativas Argentinas (ACA) - Argentina
  • Consorcio Papa - Chile

Graphics and data

Financing by country (in USD)
ArgentinaBrazilChileColombiaEcuadorParaguayUruguay050.000100.000150.000200.000250.000300.000350.000400.000450.000500.000550.000
FONTAGRO Amount Other agencies Counterpart Amount

Geolocated Map

Researchers

Project leader Argentina

Sergio Feingold

Researcher

Sabina Vidal

Researcher

Johanna Buitron

Researcher

Santiago Meneses

Researcher

Humberto Prieto

Researcher

Cecilia Pascuan

Researcher

Gabriela Massa

Researcher

Leandro Picotto

Researcher

Daniel Salamone

Researcher

Anabel Poulsen

Researcher

Federico Hozbor

Technician

Adriana Lauro

Sponsors
BID
IICA
With the support of
Fondo Coreano de Alianza para el Conocimiento en Tecnología e Innovación (KPK)